xt dna library prep kit (Illumina Inc)

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Illumina Inc xt dna library prep kit
Xt Dna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xt+library+prep+kit/Nextera+XT+DNA+Library+Preparation+Kit/pmc13015215-185-2-7
Average 99 stars, based on 11844 article reviews

xt dna library prep kit - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Multidimensional profiling of heterogeneity in supratentorial ependymomas.
Article Snippet: Next Oligo-dT primed reverse transcription was performed using Maxima H Minus reverse transcriptase (Life Technologies, EP0753) and a template-switching oligonucleotide (TSO; Qiagen) followed by PCR amplification (20 cycles for scRNA-seq and 22 cycles for snRNA-seq) using the KAPA HiFi HotStart ReadyMix (KAPA Biosystems, 07958935001), and by AMPure XP bead (Beckman Coulter, A63882) purification. .. Libraries were generated using the Nextera XT Library Prep kit (Illumina, FC-131-1096). .. Libraries from 768 cells with unique barcodes were combined and sequenced using the NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024906) on the NextSeq 500 sequencer (Illumina). scRNA-seq data processing Quality filtering.

Modification:

Article Title: Low levels of metabolic auxotrophy among environmental Pseudomonas isolates
Article Snippet: .. The genomic DNA libraries were prepared by MicrobesNG using a Nextera XT Library Prep Kit (Illumina, San Diego, USA) following manufacturer’s protocol with a modification to the DNA input (2-fold increase) and a PCR step (elongation time increased to 45 sec). .. Pipetting for DNA quantification and library preparation were done using a Hamilton Microlab STAR automated liquid handling system (Hamilton, Bonaduz AG, Switzerland).

Polymerase Chain Reaction:

Article Title: Draft Genome Sequence of Bacillus sp. Strain 11B20, a Promising Plant-Growth Promoting Bacterium Associated with Maize (Zea mays L.) in the Yaqui Valley, Mexico
Article Snippet: The extracted DNA (DNA ≥ 1 μg, concentration ≥ 20 ng/μL) was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific, Waltham, MA, USA) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd., Stevenage, Hertfordshire, UK) and diluted as appropriate [25]. .. To sequence that high-quality genomic DNA, the Illumina NovaSeq platform (2 × 250 bp) was used, and genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol, but with the following modifications: used DNA was doubled, and PCR elongation time was increased to 45 s. DNA quantification and library preparation were performed on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Bonaduz, Switzerland) [26]. .. Libraries were then sequenced on an Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) using a 250 bp paired-end protocol, and Trimmomatic version 0.30 was used to remove adapter sequences and low-quality bases, with a sliding window-quality cutoff of 15.

Article Title: Whole-genome sequencing and biosynthetic gene cluster analysis of Bacillus subtilis BAGL as a potent antifungal biocontrol agent against phytopathogenic fungi.
Article Snippet: .. Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturer’s instructions, with the following modifications: the input DNA was increased 2-fold, and the PCR elongation time was increased to 45 s. Libraries were sequenced using an Illumina NovaSeq 6000 (Illumina, San Diego, USA) with a 250 bp paired-end protocol. ..

Article Title: Draft Genome Sequence of Bacillus sp. Strain 11B20, a Promising Plant-Growth Promoting Bacterium Associated with Maize ( Zea mays L.) in the Yaqui Valley, Mexico
Article Snippet: The extracted DNA (DNA ≥ 1 μg, concentration ≥ 20 ng/μL) was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific, Waltham, MA, USA) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd., Stevenage, Hertfordshire, UK) and diluted as appropriate [ ]. .. To sequence that high-quality genomic DNA, the Illumina NovaSeq platform (2 × 250 bp) was used, and genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol, but with the following modifications: used DNA was doubled, and PCR elongation time was increased to 45 s. DNA quantification and library preparation were performed on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Bonaduz, Switzerland) [ ]. .. Libraries were then sequenced on an Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) using a 250 bp paired-end protocol, and Trimmomatic version 0.30 was used to remove adapter sequences and low-quality bases, with a sliding window-quality cutoff of 15.

Sequencing:

Article Title: Draft Genome Sequence of Bacillus sp. Strain 11B20, a Promising Plant-Growth Promoting Bacterium Associated with Maize ( Zea mays L.) in the Yaqui Valley, Mexico
Article Snippet: The extracted DNA (DNA ≥ 1 μg, concentration ≥ 20 ng/μL) was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific, Waltham, MA, USA) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd., Stevenage, Hertfordshire, UK) and diluted as appropriate [ ]. .. To sequence that high-quality genomic DNA, the Illumina NovaSeq platform (2 × 250 bp) was used, and genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol, but with the following modifications: used DNA was doubled, and PCR elongation time was increased to 45 s. DNA quantification and library preparation were performed on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Bonaduz, Switzerland) [ ]. .. Libraries were then sequenced on an Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) using a 250 bp paired-end protocol, and Trimmomatic version 0.30 was used to remove adapter sequences and low-quality bases, with a sliding window-quality cutoff of 15.

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